Definition

RNA-based NGS fusion panels screen for known and novel breakpoints in genes such as ALK, ROS1, RET, NTRK1/2/3, FGFR1-3 and MET; these fusions determine response to tyrosine kinase inhibitor therapy, particularly in NSCLC. In validation studies, concordance rates with other methods are 97% for ALK and 95% for ROS1.

Gene/region examined

ALK, BRAF, BRD4, CLIP4, EGFR, ERG, ETV1, ETV4, ETV6, EWSR1, FGFR1, FGFR2, FGFR3, MET, MYB, NOTCH2, NRG1, NTRK1, NTRK2, NTRK3, NUTM1, PDGFRA, PDGFRB, RET, ROS1, TERT, TFE3

Method

Next Generation Sequencing

Accepted sample types

Solid Tumor-FFPE

Description

75 fusion analyses across 26 gene regions.

Inheritance

Detects somatic gene fusions acquired in tumor tissue (arising from chromosomal rearrangement).

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