Anti-TPO and Anti-Tg (Thyroid Antibodies)

Definition

Measures autoantibodies directed against the thyroid gland. Three main antibodies are tested:

AntibodyWhat it indicates
TPOAb (Anti-TPO)A high level is a sign that Hashimoto's disease is causing hypothyroidism
TgAb (Anti-Tg)A high level is a sign of Hashimoto's disease
TRAb (TSH receptor antibody)May be a sign of Graves' disease

Purpose

To determine whether thyroid dysfunction, when thyroid hormone levels (T3, T4, TSH) indicate it, is due to an autoimmune cause. Symptoms that prompt the test: weight change, fatigue, tremor, heat/cold intolerance, irregular heartbeat.

Results

  • Negative, the symptoms are not of autoimmune origin
  • TPOAb and/or TgAb positive, possible Hashimoto's disease
  • TPOAb and/or TRAb positive, possible Graves' disease

The more thyroid antibodies there are, the greater the likelihood of an autoimmune thyroid disorder.

Normal Values

Laboratory-specific; refer to the reference range on your report.

Risks and Limitations

Antibody positivity is also seen in healthy people with normal thyroid function, and this is the point that most often causes concern:

In a study of 4,775 people with normal thyroid function (euthyroid) based on data from the Korea National Health and Nutrition Examination Survey:

GroupTPOAb positivity
Euthyroid people without metabolic syndrome5%
Euthyroid people with metabolic syndrome7%

In other words, about one in every 20 people whose thyroid hormones are completely normal tests positive for TPOAb.

What the patient needs to know: Anti-TPO positivity on its own does not mean "you have thyroid disease". In a person who is antibody positive but has normal TSH/T4, the diagnosis is not autoimmune thyroiditis but a state of "thyroid autoimmunity", which requires follow-up and may not require treatment.

For this reason, testing antibodies for screening purposes in people without symptoms may cause unnecessary anxiety. The test is ordered when hormone levels indicate dysfunction.

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