Difference Between a Screening Test and a Diagnostic Test

Definition

This is the most frequently confused distinction in prenatal genetics and the one that causes the most anxiety:

Screening testDiagnostic test
What it tells youRisk, "is the likelihood increased?"Definitive answer, "is it present or not?"
ExamplesNIPT (cfDNA), double/triple screeningAmniocentesis, CVS, karyotype
What a positive result meansRisk is increased, it does not mean the condition is presentThe condition is present
ProcedureBlood draw, no riskNeedle sampling, carries a low risk
What comes nextIf positive, a confirmatory test is neededNo confirmation needed

Why it matters: In cfDNA screening, a positive result means increased risk but cannot make a diagnosis; amniocentesis or CVS is needed for confirmation. In contrast, amniocentesis is a diagnostic test: it tells you whether your baby has a specific health problem, and the results are almost always accurate.

What the patient should know: The sentence "my screening test came back positive" does not mean "my baby has the condition." Families who are unaware of this distinction may go through an unnecessary and severe period of anxiety.

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